| 质粒名称: | pGL4 mIL-17 2kb promoter |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 20124 |
| 存储实验室: | Warren Strober |
|
载体骨架基本信息 |
|
| 载体名称: | pGL4.10 |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4242 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 荧光素酶表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mIL-17,mIL-17 promoter,IL-17 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 2000 bp |
| 基因突变: | -- |
| GenBank编号 | AC159614 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NheI（销毁后未知） |
| 3' 克隆位点: | BglII （销毁后未知） |
| 5' 测序引物 | RVprimer3 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGL4 mIL-17 2kb promoter was a gift from Warren Strober (Addgene plasmid # 20124 ; http://n2t.net/addgene:20124 ; RRID:Addgene_20124) |
| 参考文献部分: | Interactions between the transcription factors Runx1, RORgt and Foxp3 regulate differentiation of interleukin 17-producing T cells. Fuping Zhang, Guangxun Meng & Warren Strober. 2008 Nov;9(11):1297-306. Epub 2008 Oct 12. 10.1038/ni.1663 PubMed 18849990 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGL4 mIL-17 2kb promoter 6231 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pGL4 mIL-17 2kb promoter
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6231)
AUTHORS
TITLE Interactions between the transcription factors Runx1, RORgt and
Foxp3 regulate differentiation of interleukin 17-producing T cells
JOURNAL 2008 Nov;9(11):1297-306. Epub 2008 Oct 12.
PUBMED 18849990
REFERENCE 2 (bases 1 to 6231)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6231
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(63..80)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(234..822)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(314..333)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1022..1882)
/codon_start=1
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 1987..2035
/note="synthetic polyadenylation signal"
misc_feature 2049..2140
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 2089..2108
/label=RVprimer3
/note="pGL3 vector, forward primer"
regulatory 4223..4232
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4229..5881
/codon_start=1
/product="firefly luciferase"
/label=luciferase
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 5925..6046
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5936..5955)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 5990..6009
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
ORIGIN
1 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
961 taaagtatat atgagtaaac ttggtctgac agcggccgca aatgctaaac cactgcagtg
1021 gttaccagtg cttgatcagt gaggcaccga tctcagcgat ctgcctattt cgttcgtcca
1081 tagtggcctg actccccgtc gtgtagatca ctacgattcg tgagggctta ccatcaggcc
1141 ccagcgcagc aatgatgccg cgagagccgc gttcaccggc ccccgatttg tcagcaatga
1201 accagccagc agggagggcc gagcgaagaa gtggtcctgc tactttgtcc gcctccatcc
1261 agtctatgag ctgctgtcgt gatgctagag taagaagttc gccagtgagt agtttccgaa
1321 gagttgtggc cattgctact ggcatcgtgg tatcacgctc gtcgttcggt atggcttcgt
1381 tcaactctgg ttcccagcgg tcaagccggg tcacatgatc acccatatta tgaagaaatg
1441 cagtcagctc cttagggcct ccgatcgttg tcagaagtaa gttggccgcg gtgttgtcgc
1501 tcatggtaat ggcagcacta cacaattctc ttaccgtcat gccatccgta agatgctttt
1561 ccgtgaccgg cgagtactca accaagtcgt tttgtgagta gtgtatacgg cgaccaagct
1621 gctcttgccc ggcgtctata cgggacaaca ccgcgccaca tagcagtact ttgaaagtgc
1681 tcatcatcgg gaatcgttct tcggggcgga aagactcaag gatcttgccg ctattgagat
1741 ccagttcgat atagcccact cttgcaccca gttgatcttc agcatctttt actttcacca
1801 gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga atgagtgcga
1861 cacgaaaatg ttggatgctc atactcgtcc tttttcaata ttattgaagc atttatcagg
1921 gttactagta cgtctctcaa ggataagtaa gtaatattaa ggtacgggag gtattggaca
1981 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
2041 atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag
2101 gctgtcccca gtgcaagtgc aggtgccaga acatttctct ggcctaactg gccggtacct
2161 gagctcgcta gctacaggtg ttcaagtaac ttacaatata gaaagtaaat cagtaagcaa
2221 acaatataca ggtcaagaga aacagacagc cacataccaa agagacaaat gagtctaaag
2281 taatccatgg agctggagag atggctcagc agttaagagt actgactgct cttccagagg
2341 tcttgagttc aattcccaga aaccacatgg tggctcacaa ccatttgtaa tgggatctga
2401 taccttcttc tggtgtgtct gaagatagca tcagtgtatt cacatatatt aaataaataa
2461 atatttttta aaaagtaatc catgaagacc tatgttgtat aaaaagttat attatgtaca
2521 acaaaattta agaaaatgag taacacatta gcccataaag aagccaatgt tgtggtatgt
2581 ttattccagt gatgttattc cccgatagaa taactatttg aataggatcc tacaagtgtg
2641 ctgataaaca gggtgaaaga ggacattgcc taggctgtat tttaacagaa ggtatgtaat
2701 ttgtagaagg atgacacgta gaaagccagg acctggtgaa ccctaacatg gagcatagta
2761 acatggaggc tagaagggat gttaattcaa actgcaatac taattcaagg agttcatgct
2821 tctcattcag gaagctagag aatgggaaaa gacctggcaa tcagaggtgt gtgtgagcat
2881 tatcccaggg ataatgccaa gggtattatc ccaagggtat cccaagaagt gtcagaaaag
2941 caaacatgat ccaaacaggt gaaagtcaga gttaccagcc agcaaaagac ctagaaagaa
3001 gagcaaggtg tgaggtgctg caacttctga gaacacggtg atcatgaaca gaatccagca
3061 atcctaccag acatgccatc tattgaacag gagctatcgg tccacctcat gctgcatgtc
3121 agacaaaagc tgaagagctg ggacctaatg acccccatat tcaccatctt gtcctcatat
3181 ctgctattcc tgaagaaaaa gacttctcaa agacataaag gcaaaggtca tctcatggag
3241 aggagagaac atgagagagc tgtttccatc ttcccttctc atccctcatc tcctcctgtt
3301 agtagtctcc acccggcagt gcctcagtgt ctccactgtc tttcagcctt catcttgatt
3361 tctaattctt tcttcgattt atccaatcag tcccttattc tttcacttca tttccttcct
3421 ccttaaaaga aaggcttgat accgaacctc aaaacagcaa atattaacag gtttcttgat
3481 aacatgcaac cgtaatgact tcactagtaa acctcatgtc tctcgctact ccttaataac
3541 taactagcct ttgtgattgt ttcttgcaga gaatagacat tcaaggaaaa acagttgcgg
3601 tactcagtta aatagaacgt gttccgttgg tgttaaatta tttattttgt atgtctgttt
3661 acatactaag acattgagtg ggtttctttg ggcaagggat gctctctagc cagggaattt
3721 ggtagaaaag tgagaaagat caagtcaaaa ttcaaagtgt gtgtcactag gagactgtca
3781 agagactcac aaaccattac tatggagccc agctctgcag cagcttcaga tatgtccata
3841 cacacatgat actgaatcac agcaaagcat ctctgttcag ctcccaagaa gtcatgcttc
3901 tttgcatagt gaacttctgc ccttcccatc taccttcgag acagatgttg cccgtcataa
3961 aggggtggtt ctgtgctgac ctcatttgag gatggaatct ttactcaaat ggtgtcaccc
4021 cccaacccac tcttgacgta agtgaccaca gaggtagtaa aaccgtataa aaagagagaa
4081 aggagcacta ctcttcatcc acctcacacg aggcacaagt gcacccagca ccagctgatc
4141 aggacgcgca aacatgagtc cctcgaggat atcaagatct ggcctcggcg gccaagcttg
4201 gcaatccggt actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc
4261 agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa
4321 gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat
4381 tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg
4441 gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc
4501 cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga
4561 gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa
4621 agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat
4681 catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca
4741 tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac
4801 catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc
4861 gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat
4921 catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac
4981 cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga
5041 gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact
5101 atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga
5161 gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt
5221 ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat
5281 cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc
5341 taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg
5401 cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct
5461 catcgacaag gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca
5521 cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc
5581 agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg
5641 cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa
5701 aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa
5761 gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga
5821 cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta
5881 ataattctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
5941 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
6001 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
6061 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
6121 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
6181 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca c
//
